Enzymes are biological catalysts. Relate the chemical structure of an enzyme to its specificity and catalytic activity. Design a quantitative experiment to investigate the influence of pH or temperature on the activity of an enzyme. Describe what information concerning the structure of an enzyme could be inferred from your experiments.

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Enzymes are biological catalysts. Relate the chemical structure of an enzyme to its specificity and catalytic activity.  Design a quantitative experiment to investigate the influence of pH or temperature on the activity of an enzyme.  Describe what information concerning the structure of an enzyme could be inferred from your experiments.


  1. For an enzyme to function, there must be enzyme/substrate speceficity. This includes the intermolecular forces between the amino acids of the enzyme which contribute to the shape of the folded enzyme.
  2. Use liver catalase, which breaks down hydrogen peroxide.

    independent variable-pH

    dependent variable-amount of products produced by reaction (measures the ideality of the conditions of the reaction)

    control variables-temperature of the room, the concentration of enzyme, the concentration of hydrochloric acid, the volume of enzyme and hydrochloric acid solution alter pH by adding drops of acid (HCL) or base (KOH) to a set volume of liver (blended).
  3. The effectiveness of the reaction is measured based on how much product, bubbles, were produced. IMore products is better, means the pH was closer to optimal pH. No product means enzyme was denatured. 
  4. You can infer the optimal pH of enzyme, and at what pH it has become denatured

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