Enzymes are biological catalysts. Relate the chemical structure of an enzyme to its specificity and catalytic activity. Design a quantitative experiment to investigate the influence of pH or temperature on the activity of an enzyme. Describe what information concerning the structure of an enzyme could be inferred from your experiments.

-

Enzymes are biological catalysts. Relate the chemical structure of an enzyme to its specificity and catalytic activity.  Design a quantitative experiment to investigate the influence of pH or temperature on the activity of an enzyme.  Describe what information concerning the structure of an enzyme could be inferred from your experiments.


  1. For an enzyme to function, there must be enzyme/substrate speceficity. This includes the intermolecular forces between the amino acids of the enzyme which contribute to the shape of the folded enzyme.
  2. Use liver catalase, which breaks down hydrogen peroxide.

    independent variable-pH

    dependent variable-amount of products produced by reaction (measures the ideality of the conditions of the reaction)

    control variables-temperature of the room, the concentration of enzyme, the concentration of hydrochloric acid, the volume of enzyme and hydrochloric acid solution alter pH by adding drops of acid (HCL) or base (KOH) to a set volume of liver (blended).
  3. The effectiveness of the reaction is measured based on how much product, bubbles, were produced. IMore products is better, means the pH was closer to optimal pH. No product means enzyme was denatured. 
  4. You can infer the optimal pH of enzyme, and at what pH it has become denatured

Comments

Post a Comment

Popular posts from this blog

Discuss the seven characteristics of useful information.

-
Discuss the seven characteristics of useful information. Answer: The seven characteristics of useful information are: relevant, reliable, complete, timely, understandable, verifiable and accessible. These characteristics are qualities that information should possess to be useful in a business environment. Briefly stated, in order for information to be useful it must be: 1) relevant, meaning that it reduces uncertainty and adds to the decision-making process; 2) reliable information is information that is free from error, and is accurate in its nature; 3) complete information is information that does not omit any important data, facts, or aspects about events or activities; 4) information is timely when it is fully available to enable the decision-making process to proceed; 5) understandable information must be both in an intelligible and useful format; 6) information is considered verifiable if two people, acting independently of each other, produce the same inform
Read more

Why have accounting software packages been designed with separate transaction modules?

-
Why have accounting software packages been designed with separate transaction modules? Answer: : Since every organization does not necessarily use all of the transaction cycles in its operations, it is to the advantage of the organization to be able to "pick and choose" from among various software modules that track and record different transaction cycles. For example, a law firm would have no need to implement a production cycle module. Also, the nature of a transaction cycle varies across the broad spectrum of business organizations. Again, a law firm would have a revenue cycle, but it would not involve the purchase, receipt, and payment for products or merchandise; likewise a retail store chain may not sell any consulting services to its customers.
Read more

A laboratory assistant prepared solution of 0.8 M, 0.6 M, 0.4 M, and 0.2 M sucrose, but forgot to label them. After realizing the error, the assistant randomly labeled the flasks containing these four unknown solutions as flask A, flask B, flask C, and flask D.

-
A laboratory assistant prepared solution of 0.8 M, 0.6 M, 0.4 M, and 0.2 M sucrose, but forgot to label them. After realizing the error, the assistant randomly labeled the flasks containing these four unknown solutions as flask A, flask B, flask C, and flask D. Design an experiment, based on the principles of diffusion and osmosis, that the assistant could use to determine which of the flasks contains each of the four unknown solutions. Include in your answer (a) a description of how you would set up and perform the experiment: (b) the results you would expect from your experiments: and (c) an explanation of those results based on the principles involved. (Be sure to clearly state the principles addressed in your discussion.)
Read more