A laboratory assistant prepared solution of 0.8 M, 0.6 M, 0.4 M, and 0.2 M sucrose, but forgot to label them. After realizing the error, the assistant randomly labeled the flasks containing these four unknown solutions as flask A, flask B, flask C, and flask D.
A laboratory assistant prepared solution of 0.8 M, 0.6 M, 0.4 M, and 0.2 M sucrose, but forgot to label them. After realizing the error, the assistant randomly labeled the flasks containing these four unknown solutions as flask A, flask B, flask C, and flask D.
Design an experiment, based on the principles of diffusion and osmosis, that the assistant could use to determine which of the flasks contains each of the four unknown solutions. Include in your answer
(a) a description of how you would set up and perform the experiment:
(b) the results you would expect from your experiments: and
(c) an explanation of those results based on the principles involved. (Be sure to clearly state the principles addressed in your discussion.)
(a) First I would pour an equal volume of each solution into beakers labeled A, B, C, and D. I would also have a beaker of the same amount of distilled water to serve as a control for the experiment. Then I would obtain several baby carrots, 4 per beaker. I would mass the groups of 4 carrots before placing them in their respective beakers of solution. Carrots do have semipermeable cell membranes (water should be able to pass, but not necessarily the larger sucrose), but in order to allow materials to pass through the carrots and be able to measure a change I would let the carrots sit overnight (or at least for several hours after placing them in the solutions). Upon returning to the lab, I would extract the carrots from their solutions, blot them dry, mass them again, and then calculate the percent change in mass of the carrots after sitting in solution.
(b) I would expect that the carrots in water as well as possibly the 0.2 M sucrose would gain mass. The carrots in 0.4 M, 0.6M and 0.8M sucrose solution should show greater percentage mass loss with each increasing concentration of sucrose in solution.
(c) The results seen in the water control as well as possibly the 0.2M sucrose occur because the water potential outside of the carrots would be higher thus encouraging water to move into the carrots and increase their mass due to osmosis.
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