Define restriction enzymes, and outline how they are used to make recombinant DNA.

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Define restriction enzymes, and outline how they are used to make recombinant DNA.



Restriction Enzymes: DNA cutting enzymes that exist in many bacteria. Very specific in their fxn and action. Some produce "sticky" ends, which are short stretches of single-stranded DNA at the ends of the DNA fragements.
-Fragments of DNA production by the same restriction enzyme will spontaneously join by hydrogen bonding(thus "sticky")
-DNA ligase is an enzyme that can covalently link the DNA backbones.

-Restriction enzymes take apart the DNA in a certain area and allow for a plasmid to be inserted within the gap that is created

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